Melanostaining is a specialized protocol designed to assess melanin content and distribution in skin models following treatment. It begins with Fontana-Masson staining, a silver-based histological technique that selectively highlights melanin granules as black deposits, enabling precise visualization of pigmentation.
Following staining, Melanostaining integrates image quantification to evaluate changes in melanin synthesis and localization. This comprehensive approach allows researchers to measure the efficacy of active ingredients targeting pigmentation, whether for brightening, photoprotection, or anti-aging purposes.
Melanostaining offers a robust and reproducible framework for studying pigmentation modulation in both normal and stressed skin conditions.